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anti p pdk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p pdk
    Anti P Pdk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+pdk1/Phospho-PDK1+(Ser241)+Rabbit+mAb/pm41608625-82-51-54
    Average 95 stars, based on 209 article reviews
    anti p pdk - by Bioz Stars, 2026-08
    95/100 stars

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    Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of <t>PDK1</t> <t>(Phospho-Ser241),</t> PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.
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    Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of <t>PDK1</t> <t>(Phospho-Ser241),</t> PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.
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    Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of <t>PDK1</t> <t>(Phospho-Ser241),</t> PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.
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    anti phospho pdk1 ser241 rabbit monoclonal ab  (Cell Signaling Technology Inc)
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    PZ-DHA inhibits <t>PDK1</t> and mTOR phosphorylation in HUVECs. HUVECs were treated with PZ, DHA, PZ-DHA (10 μM), or vehicle alone and cultured for 72 h. Cells were harvested and protein-rich cell lysates were prepared for western blot analysis of ( A ) phospho-PDK1 <t>(Ser241)/total-PDK1</t> expression or ( B ) phospho-mTOR (Ser2448)/total-mTOR expression. Equal protein loading was confirmed by β-actin expression. Data shown are mean % relative expression ± SEM from 3 independent experiments. The ANOVA multiple means comparison statistical method was performed and differences among means were compared using Tukey’s test; ** p < 0.01, *** p < 0.001. (Original Western Blot Images see ).
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    PZ-DHA inhibits <t>PDK1</t> and mTOR phosphorylation in HUVECs. HUVECs were treated with PZ, DHA, PZ-DHA (10 μM), or vehicle alone and cultured for 72 h. Cells were harvested and protein-rich cell lysates were prepared for western blot analysis of ( A ) phospho-PDK1 <t>(Ser241)/total-PDK1</t> expression or ( B ) phospho-mTOR (Ser2448)/total-mTOR expression. Equal protein loading was confirmed by β-actin expression. Data shown are mean % relative expression ± SEM from 3 independent experiments. The ANOVA multiple means comparison statistical method was performed and differences among means were compared using Tukey’s test; ** p < 0.01, *** p < 0.001. (Original Western Blot Images see ).
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    Image Search Results


    Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of PDK1 (Phospho-Ser241), PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.

    Journal: Drug Design, Development and Therapy

    Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

    doi: 10.2147/DDDT.S513643

    Figure Lengend Snippet: Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of PDK1 (Phospho-Ser241), PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.

    Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

    Techniques: Ab Array

    ZXF regulated the PI3K/PDK1/AKT/FoxO1 signaling pathway. (n=5). ( A ) Western blot of phosphorylated and total protein levels of PI3K, PDK1, AKT, and FOXO1. ( B – E ) Statistical results of p-PI3K/PI3K, p-PDK1/PDK1, p-AKT/AKT, and p-FOXO1/FOXO1. ** P <0.01 vs Con group; Δ P <0.05, ΔΔ P <0.01 vs Dox group.

    Journal: Drug Design, Development and Therapy

    Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

    doi: 10.2147/DDDT.S513643

    Figure Lengend Snippet: ZXF regulated the PI3K/PDK1/AKT/FoxO1 signaling pathway. (n=5). ( A ) Western blot of phosphorylated and total protein levels of PI3K, PDK1, AKT, and FOXO1. ( B – E ) Statistical results of p-PI3K/PI3K, p-PDK1/PDK1, p-AKT/AKT, and p-FOXO1/FOXO1. ** P <0.01 vs Con group; Δ P <0.05, ΔΔ P <0.01 vs Dox group.

    Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

    Techniques: Western Blot

    Illustration of the PI3K/PDK1/AKT/FoxO1 Signaling Pathway in Inhibiting Cardiomyocyte Apoptosis and the Role of ZXF.

    Journal: Drug Design, Development and Therapy

    Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

    doi: 10.2147/DDDT.S513643

    Figure Lengend Snippet: Illustration of the PI3K/PDK1/AKT/FoxO1 Signaling Pathway in Inhibiting Cardiomyocyte Apoptosis and the Role of ZXF.

    Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

    Techniques:

    Antibodies and dilutions used for Western blot analysis

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: One day of environment-induced heat stress damages the murine myocardium

    doi: 10.1152/ajpheart.00180.2024

    Figure Lengend Snippet: Antibodies and dilutions used for Western blot analysis

    Article Snippet: Phospho-PDK1 (S241) (C49H2) , CST, No. 3438 , 1:1,000 , 1:2,000.

    Techniques: Western Blot

    Effect of environment-induced heat stress (EIHS) on mammalian target of rapamycin (mTOR) signaling. EIHS increased phosphoinositide-dependent protein kinase 1 (PDK1), phospho (p)-PDK1, p-AKT, and p-mTOR compared with thermoneutral (TN). Total AKT, total mTOR, and total p70 S6 kinase (p70S6K), phosphorylated p70S6K, total initiation factor 4E binding protein (4EBP1) and p-4EBP1 were similar between groups. Results are expressed as means ± SD. * P < 0.05; n = 8 or 9/group for PDK1 and p-PDK1. All other measures used smaller gels such that n = 6/group. One outlier was removed from TN PKD1.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: One day of environment-induced heat stress damages the murine myocardium

    doi: 10.1152/ajpheart.00180.2024

    Figure Lengend Snippet: Effect of environment-induced heat stress (EIHS) on mammalian target of rapamycin (mTOR) signaling. EIHS increased phosphoinositide-dependent protein kinase 1 (PDK1), phospho (p)-PDK1, p-AKT, and p-mTOR compared with thermoneutral (TN). Total AKT, total mTOR, and total p70 S6 kinase (p70S6K), phosphorylated p70S6K, total initiation factor 4E binding protein (4EBP1) and p-4EBP1 were similar between groups. Results are expressed as means ± SD. * P < 0.05; n = 8 or 9/group for PDK1 and p-PDK1. All other measures used smaller gels such that n = 6/group. One outlier was removed from TN PKD1.

    Article Snippet: Phospho-PDK1 (S241) (C49H2) , CST, No. 3438 , 1:1,000 , 1:2,000.

    Techniques: Binding Assay

    PZ-DHA inhibits PDK1 and mTOR phosphorylation in HUVECs. HUVECs were treated with PZ, DHA, PZ-DHA (10 μM), or vehicle alone and cultured for 72 h. Cells were harvested and protein-rich cell lysates were prepared for western blot analysis of ( A ) phospho-PDK1 (Ser241)/total-PDK1 expression or ( B ) phospho-mTOR (Ser2448)/total-mTOR expression. Equal protein loading was confirmed by β-actin expression. Data shown are mean % relative expression ± SEM from 3 independent experiments. The ANOVA multiple means comparison statistical method was performed and differences among means were compared using Tukey’s test; ** p < 0.01, *** p < 0.001. (Original Western Blot Images see ).

    Journal: Biomolecules

    Article Title: Phloridzin Docosahexaenoate, an Omega-3 Fatty Acid Ester of a Flavonoid Precursor, Inhibits Angiogenesis by Suppressing Endothelial Cell Proliferation, Migration, and Differentiation

    doi: 10.3390/biom14070769

    Figure Lengend Snippet: PZ-DHA inhibits PDK1 and mTOR phosphorylation in HUVECs. HUVECs were treated with PZ, DHA, PZ-DHA (10 μM), or vehicle alone and cultured for 72 h. Cells were harvested and protein-rich cell lysates were prepared for western blot analysis of ( A ) phospho-PDK1 (Ser241)/total-PDK1 expression or ( B ) phospho-mTOR (Ser2448)/total-mTOR expression. Equal protein loading was confirmed by β-actin expression. Data shown are mean % relative expression ± SEM from 3 independent experiments. The ANOVA multiple means comparison statistical method was performed and differences among means were compared using Tukey’s test; ** p < 0.01, *** p < 0.001. (Original Western Blot Images see ).

    Article Snippet: Anti-CDK4 rabbit monoclonal Ab, anti-cyclinD3 rabbit monoclonal Ab, anti-total- PDK1 rabbit monoclonal antibody (Ab), anti-phospho-PDK1 (Ser241) rabbit monoclonal Ab, anti-total-mTOR rabbit monoclonal Ab, anti-phospho-mTOR (Ser2448) rabbit monoclonal Ab, anti-RhoA rabbit monoclonal Ab, anti-Rac1/2/3 rabbit monoclonal Ab, anti-Cdc42 rabbit monoclonal Ab, HRP-conjugated rabbit anti-β-actin, and HRP-conjugated donkey anti-rabbit Ab were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA).

    Techniques: Phospho-proteomics, Cell Culture, Western Blot, Expressing, Comparison